ODMR Analysis#
Prerequisites
Beginner lessons
A local copy of this lesson’s assets, found here
Objectives
Improve upon a plotting analysis pipeline
Learn about plotting Pandas arrays
This lesson provides sample data and investigative Jupyter notebook. You may
follow along with the ODMR_Analysis.ipynb
as a supplement to this lesson.
Introduction#
Optically detected magnetic resonance (ODMR) spectroscopy is a way to detect materials that have a singlet-state to triplet-state transition. When such a material is excited, some relaxation pathways occur through the emission of photons, which can be characterized with a spectrometer. The majority of emission occurs at a predominant spectral line known as the Zero Phonon Line (ZPL), but intersystem crossing allows for less-stable and more sparsely-occurring radiative pathways at longer wavelengths.
The difference in energy between a singlet-state and a triplet-state is associated with photons of microwave frequencies (GHz). When the system is pumped with a microwave signal that matches this transition, the relaxation pathway associated with intersystem crossing is temporarily enhanced and competes with the relaxation pathway responsible for ZPL emission. Sweeping the microwave field through a range of frequencies results in characteristic dips in the observed fluorescence of the ZPL. At larger magnetic fields, the separation of these dips becomes more prominent due to the Zeeman splitting of the triplet states.
Initial Analysis#
Our colleague gathered some ODMR spectra and handed it to us for analysis. Specifically, he wants to identify the frequencies for which the ZPL signal is reduced.
The measurement consists of a ZPL signal continuously excited by a laser source in the presence of various driving microwave frequencies (delivered as microwave pulses). The ZPL signal is represented by counts in a photon detector collected for a given amount of time, measured both before and after the microwave pulse is applied (labeled Reference and Signal, respectively). By comparing the photoluminescence (PL) signal before and during the microwave pulse, one can determine the relative dip in PL signal. By repeating this measurement for a range of microwave frequencies, one can identify where the ZPL signal is most reduced.
Our colleague kept track of user-specific parameters like the number of
microwave pulses over which the PL signal was integrated, the excitation laser
power, and the time delay between when the pulse was applied compared to when
the photoluminescence measurement began. You will find these in the data/
folder with names like ODMR_500kBurst_350uW_50nsdelay.csv
. In reality, there
might be many such iterations to inspect, but a only a down-selected number of
files is presented for this lesson.
Files with similar acquisition parameters may be combined in one analysis. The number of microwave pulses in each acquisition is largely irrelevant except for if you want to evaluate the efficiency of the reduction in PL. The purpose of probing multiple microwave pulses is to help resolve contrast between a faint signal and background noise. Integrating over more pulses takes longer to acquire, but typically results in higher signal-to-noise ratio.
If after one ODMR spectral acquisition the contrast in PL is unclear, we can repeat the measurement and combine with the previous acquisition to effectively increase the number of pulses over which we integrate.
Understanding our colleague’s initial analysis#
Our colleague investigated his ODMR contrast after each acquisition. He found
that after one acquisition, the ODMR contrast did not show up well in a graph,
so he repeated the same acquisition a number of times. He copied the results of
subsequent acquisitions into an Excel file and accumulated the Reference and
Signal values from all identical acquisitions before determining a composite
ODMR contrast. This analysis appears in an Excel file alongside the first
acquisition, but was later converted to a .csv
for better compatibility with
Dropbox.
This approach has several problems:
Data and analysis are not logically separated
It is not clear by filename alone which acquisition houses the analysis
All equations in the original
.xlsx
file are lost during the conversion to.csv
Inclusion of further measurements means recreating the original
.xlsx
equations and updating the corresponding analysis.
The analysis in Excel is simple enough to recreate, but let’s practice framing this in Python.
Refined Analysis#
We’ll set out with the following objectives:
Leave original data alone
Generate and save our ODMR contrast figure to a specified directory
We’ll also specify some assumptions about what we should expect from our end user and what we promise to do in return.
Guidelines#
The user is expected to provide a list of files of ODMR spectra collected at the same location and with identical power settings. The filenames can differ in number of microwave bursts, but currently the number of integrations is not tracked in the dataframe. The ODMR data is concatenated for all files in this list, so extending the integration is as simple as adding another file to the list.
Data requirements#
Files in list have identical structure [Frequency, Ref, Sig]
Frequency values in each file have an overlapping subset
Frequency values are between 0 and 65e3 (uint16)
APD counts from all accumulated runs do not total more than 4.2e9 (uint32) in either channel
Usage:#
Adjust the filename list to point to the directory where your ODMR files are located
If analysis is needed on only one file, pass in a list of one element.
Select appropriate filtering in
glob
to pick out a subset of files if desiredAdjust threshold and distance parameters for cleaning via
scipy.signal.find_peaks
Run All Cells
Inspect cleaning results, adjust threshold as necessary
Grab figures as necessary for publication
Adjust graph style with rcParams instead of in plot